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. 2019 Jul 31;33(11):12036–12046. doi: 10.1096/fj.201900016RR

Figure 1.

Figure 1

Scratching and expression of IL-6 and p-ERK in mice injected with CaP. A) The number of hind paw scratches was counted for 2 h after intradermal injection of CaP (0, 0.5 and 5 mg/ml). B) The level of IL-6 in the dorsal skin of ICR mice injected with or without CaP was measured with ELISA. C) Western blot analysis of total ERK (t-ERK 1/2), p-ERK 1/2 and GAPDH in DRG of mice injected with/without CaP. D, E) The ratios of intensities of p-ERK 1/2 (D) and t-ERK 1/2 (E) relative to GAPDH in Western blot analysis were shown. The mean ± se for 3 independent experiments with 4 mice per group was calculated. Ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001.