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. 2019 Aug 20;33(11):12392–12408. doi: 10.1096/fj.201900854R

Figure 2.

Figure 2

AECII apoptosis in aged LC3B−/− mice. A) Western blot analysis of lung homogenates of 13-wk-old (left) or 42-wk-old (right) LC3B−/− and WT mice for cleaved caspase-3 and β-actin. B) Densitometry analysis of cleaved caspase-3/β-actin ratio. C, D) Representative immunohistochemistry images of serial lung sections of 42-wk-old LC3B−/− and WT mice for cleaved caspase-3 and proSP-C. Original magnification of images are ×200 (C) and 400, (D). Scale bars, 50 and 25 μm, respectively. Arrows in D indicate apoptotic AECII in LC3B−/− mice. E) Immunohistological staining for cleaved caspase-3 in several regions of the lungs were quantified and depicted as a bar graph. Representative blots and analysis from n = 5 mice/group and ≥3 independent experiments are shown. **P < 0.01.