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. 2019 Aug 26;33(11):12541–12553. doi: 10.1096/fj.201901213R

Figure 1.

Figure 1

Specific depletion of Sin3a leads to developmental failure during the morula-to-blastocyst transition. A) Validation of KD efficiency of Sin3a siRNAs by qPCR. In vivo–derived mouse zygotes were microinjected with Sin3a siRNAs (20 µM; cKD) or NC. Embryos were collected at morula stage (5–10 embryos per pool; n = 3) for qPCR against Sin3a and Sin3b, a homolog of Sin3a. B) Immunostaining validation of Sin3a KD efficiency (n = 3; 5–10 embryos were analyzed/group/replicate). Scale bar, 25 µm. C) Quantification of Sin3a signal intensity for the experiment described in B. D) Representative photos of NC and KD embryos from E1.5 to E4.25. Asterisk indicates abnormal embryos; arrowhead denotes degenerating embryos. E) Blastocyst rate in NC and KD groups at E4 (n = 3). Data are presented as means ± sem. *P < 0.05. F) Total cell number per embryo was reduced from E2.75 in KD groups (n = 3; P < 0.05).