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. 2019 Aug 26;33(11):12541–12553. doi: 10.1096/fj.201901213R

Figure 4.

Figure 4

Sin3a depletion leads to a failure of the first lineage specification event. A) qPCR analysis of key lineage-specific genes (TE: Cdx2, Tead4, and Yap1; ICM: Oct4 and Nanog) in morulae (n = 3 pools of 5–10 embryos/group). B–D) Immunocytochemical analysis of Cdx2 and Nanog in morulae and blastocysts after RNA interference. The intensity of Cdx2 and Nanog was diminished in KD (n = 3 pools of 5–10 embryos/group). *P < 0.05. E) Normal distribution pattern of E-cadherin and Ctnnb1, 2 adhesion molecules, in KD morulae. F) No difference was detected for Yap1 and phosphorylated Yap (pYap) between NC and KD morulae.