Figure 2.

Effects of IL‐6, INF‐α2a, and phenobarbital (PB) on STAT1 mRNA expression in primary human hepatocytes. The hepatocyte‐Kupffer cell cocultures used to determine levels of P450 enzymes (N = 3, experiment in cells from each donor was conducted once) were incubated for 72 hour with medium alone, medium containing IL‐6 (2, 10, or 50 ng/mL), INF‐α2a (0.1, 0.5, or 2.5 ng/mL), or PB (750 µmol/L). The mRNA levels were quantitated by qPCR with normalization to glyceraldehyde 3‐phosphate dehydrogenase or 18S mRNA and to the mRNA levels of the STAT1 in the medium control cultures. The normalization to 18S mRNA was performed to match conditions used by Chen et al.6 Data were normalized to medium control cultures values set to equal 1. Patterned bars indicate mRNA above 2‐fold of the control. Asterisks (*) indicate difference from the control with one‐tailed t test, P < .05 (SigmaPlot™ 12.5, Systat Software, Inc)