Figure 2.

Eeyarestatin in combination with either sunitinib or vandetanib synergistically induce cell death. A, Cleaved caspase 3–positive and cleaved PARP–positive cells were quantified using flow cytometry after treatment of TT cells or TT-shRNA-ATF4 clone #74, #76 with eeyarestatin alone(4μM), vandetanib alone(4μM), sunitinib (4μM) alone, or their combinations for 24 h. B, Cumulative analysis of cell death as described in A from three independent experiments. The data presented as mean± SD. The statistical analysis used was a two-way analysis of variance (Tukey's multiple comparisons test). *P < 0.05, **P < 0.01, ****P < 0.0001. C, Western blot showing levels of apoptotic and survival proteins in control cells and cells treated for 48 h as indicated. Vinculin served as a loading control. Con, control non treated cells; EE, eeyarestatin; SU, sunitinib, Van, vandetanib