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. 2019 Sep 13;1(10):609–623. doi: 10.1096/fba.2019-00048

Figure 1.

Figure 1

Acute exposure to inhaled e‐cig aerosols containing nicotine increases inflammatory cellular influx in BALF. Mice were exposed to air, PG, and PG with nicotine (PG+Nicotine) for 3 days (2 h/d). Mice were sacrificed 2 hours post final exposure on day 3. Total cell counts per milliliter in BALF were determined by trypan blue staining using Bio‐Rad Tc10 cell counter. Differential cells: F4/80+ macrophages, LY6B.2+ neutrophils, CD4a+ T lymphocytes, and CD8a+ T lymphocytes were determined using flow cytometry, combined results (A), male (B) and female (C) are presented individually. (D). MPO activity in BALF. Data are shown as mean ± SEM (n = 6/group; equal number of male and female mice were used; n = 3 for male and female groups respectively; *P <0.05, **P < 0.01, ***P < 0.001 significant compared with air‐exposed control; # P < 0.05, ## P < 0.01, ### P < 0.001 significant compared with PG exposed group.)