Figure 4.

Acute exposure to inhaled e‐cig aerosols containing PG with or without nicotine leads to increased levels of nicotinic acetylcholine receptors in mouse lungs. The protein abundance of nicotinic acetylcholine receptors was measured in lung homogenates using western blotting. β‐actin was used as an endogenous control. Representative blots for nAChR α3 and nAChR α7 levels in air, PG and PG with nicotine exposed male (A) and female (B) mice are shown. (C) The band intensity was measured by densitometry and data are shown as fold change relative to air group control; β‐actin was used as a loading control. Data are shown as mean ± SEM (n = 6/group; equal number of male and female mice were used; n = 3 for male and female groups respectively; *P < 0.05 significant compared with air‐exposed control)