Skip to main content
. 2019 Jul 9;1(8):498–510. doi: 10.1096/fba.2019-00011

Figure 6.

Figure 6

Podocyte GSK3α knockdown is detrimental causing increased urinary albumin creatinine ratio in vivo and increased cell death with impaired autophagic flux in ciGSK3αKO cells. A, Urinary albumin:creatinine is increased in podGSK3αKD mice. Unpaired t test, *P < 0.05. n = 12‐13 mice per group. B, Cell survival assay showing 25% cell death in ciGSK3αKO podocytes 7 d after transduction when compared with untransduced control cells or Cre lenti transduced wild‐type podocytes. ANOVA with Tukey's post hoc test, **P < 0.01. wild‐type cells, n = 3, GSK3α floxed cells n = 6 experiments. C, Representative blots of ciGSK3αKO and control cells treated with bafilomycin A1 for 4 h. D, Densitometry showing increased basal expression of p62 in ciGSK3αKO cells. Unpaired t test, *P < 0.05 n = 3. E, Level of p62 degradation represented by the difference in p62 levels ± bafilomycin A1 in ciGSK3αKO and control cells. Unpaired t test, *P < 0.05 n = 4. F, Accumulation of LC3II represented by the difference in LC3II levels ± bafilomycin A1 in ciGSK3αKO and control cells. Unpaired t test, *P < 0.05 n = 3