KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| B220-PerCP Cy5.5 (monoclonal) | Biolegend | Cat# 103236; RRID:AB_893354 |
| TNFα PerCP Cy5.5 (monoclonal) | Biolegend | Cat# 506322; RRID:AB_961434 |
| CD4 PerCP Cy5.5 (monoclonal) | Biolegend | Cat# 100433; RRID:AB_893330 |
| VDAC AF488 (monoclonal) | Abcam | Cat# ab179839 |
| IFNγ BV510 (monoclonal) | Biolegend | Cat# 505842; RRID:AB_2734494 |
| PD1 BV786 (monoclonal) | Biolegend | Cat#135225; RRID:AB_2563680 |
| NK1.1 APC (monoclonal) | BD pharmigen | Cat# 561117; RRID:AB_10563422 |
| Tim3 PE (monoclonal) | Biolegend | Cat# 119703; RRID:AB_345377 |
| Ki67 BV605 (monoclonal) | Biolegend | Cat# 652413; RRID:AB_2562664 |
| p-p38MAPK (T180/Y182) – PE (monoclonal) | Cell Signaling | Cat# 6908S; RRID:AB_10839411 |
| p-NF-kappaB p65 (S536) - A647 (monoclonal) | Cell Signaling | Cat# 4887S; RRID:AB_561198 |
| pSTAT3 (Tyr705) BV421 (monoclonal) | Biolegend | Cat# 651009; RRID:AB_2572087 |
| CD127 (IL7Ra) PE- Cy7 (monoclonal) | Biolegend | Cat# 135014; RRID:AB_1937265 |
| CD62L BV786 (monoclonal) | BD Horizon | Cat# 564109; RRID:AB_2738598 |
| KLRG1 APC-Cy7 (monoclonal) | Biolegend | Cat# 138426; RRID:AB_2566554 |
| TCF7/TCF1 FITC (monoclonal) | R&D systems | Cat# IC8224G |
| Foxp3 AxF700 (monoclonal) | Thermo Fisher | Cat# 56-5773-82; RRID:AB_1210557 |
| Leptin R biotinylated (mouse polyclonal) | R&D systems | Cat# BAF497; RRID:AB_2296953 |
| Mouse Leptin (polyclonal Goat) | R&D systems | Cat # AF498; RRID:AB_355394 |
| Phospho- ATF-2 (Thr71) (rabbit monoclonal) | Cell Signaling | Cat# 24329 |
| P38 MAPK (rabbit monoclonal) | Cell Signaling | Cat# 9212S; RRID:AB_330713 |
| InVivo Mab Anti-mouse CD8α(YTS 169.4) | BioXCell | BE0117; RRID:AB_10950145 |
| InVivoMAb rat IgG1 Isotype control | BioXCell | BE0290; RRID:AB_2687813 |
| β-Actin (C4) (mouse monoclonal) | Santa Cruz | Cat# SC-47778; RRID:AB_2714189 |
| Bacterial and Virus Strains | ||
| wild-type Vaccinia virus Western Reserve (WR) | American Type Culture Collection (BEI Resources) | Cat# NR2639 |
| One Shot® Stbl3™ Chemically Competent E. coli | Fisher | Cat# C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2NBDG | Cayman Chemical | Cat# 186689-07-6 |
| Mitotracker™ Deep Red FM | Invitrogen | Cat# M22426 |
| Tetramer H-2Kb TRP-2 PE | MBL International | Cat# TB-5004-1 |
| Tetramer VACV B8R BV421 | MBL International | Cat# BTB-M538 |
| Recombinant mouse Leptin | R&D systems | Cat# 498-OB-01M |
| L-glutamine | Fisher | Cat# MT25005CI |
| Oligomycin Complex | Cayman Chemical | Cat# 11341 |
| FCCP | Cayman Chemical | Cat# 15218 |
| 2-deoxy-D-glucose (2DG) | Sigma | Cat# D8375-5G |
| Rotenone | Sigma | Cat# R8875-5G |
| Antimycin A | Sigma | Cat# A8674-25MG |
| Recombinant Murine IL-2 | Peprotech | Cat# 212-12 |
| SIINFEKL peptide | AnaSpec | Cat# AS-60193-1 |
| Deposited Data | ||
| Raw and analyzed data SC-RNAseq | This paper | GEO: GSE133699 |
| Experimental Models: Cell Lines | ||
| CL24 | Najjar et al., 2019 | N/A |
| MC38 | Gift from Dr. Dario Vignali (University of Pittsburgh) | N/A |
| PanCO2 | Gift from Dr. Michael Lotze (University of Pittsburgh) | N/A |
| Experimental Models: Organisms/Strains | ||
| B6.129P2-Leprtm1Rck | Jackson Laboratory | Stock# 008327 |
| Ptenf/fBrafV600ETyrCre.ER | Jackson Laboratory | Stock # 013590 |
| B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ | Jackson Laboratory | Stock # 022071 |
| Recombinant DNA | ||
| Mouse Leptin natural ORF mammalian (pPCMV3-mLEP) | Sino Biological Inc. | Cat# MG50442-UT |
| PCMV3-untagged Negative Control Vector | Sino Biological Inc. | Cat# CV011 |
| pSC65 plasmid | Kind gift from Prof. Bernie Moss, NIH | N/A |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software, Inc | https://www.graphpad.com/demos/ |
| BD FACS Diva Sotware | BD Biosciences | |
| FlowJo version 10 | TreeStar, Ahsland Oregon | https://www.flowjo.com |
| Seahorse Wave Desktop Software | Agilent | https://www.graphpad.com/demos/ |
| Cell Ranger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Scanpy | Wolf et al., 2018 | https://github.com/theislab/scanpy |
| Critical Commercial Assays | ||
| 10X Genomics | 10X Genomics, Pleasanton, CA | https://www.10xgenomics.com/ |
| Seahorse XFe96 Analyzers | Agilent | https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers/seahorse-xfe96-analyzer |