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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: Biochemistry. 2018 Nov 1;57(45):6416–6433. doi: 10.1021/acs.biochem.8b00732

Table 1.

Key spectroscopic results for the assignments of the H-heme and L-heme in NeCcP.

Heme Data NeCcP, DF NeCcP, SR
High potential,
H-heme		 Oxidation State Ferric Ferrous
Spin State LS LS/HSa
EPR data g = 3.41 Disappearance of g = 3.41
rRaman data 1364 (ν4), 1504 (ν3), 1640 (ν10) 1351 (ν4), 1472 (ν3), 1492 (ν3)
MCD data 25130 cm−1,24050 cm−1 22860 cm−1, 18160 cm−1
Low-potential,
L-heme		 Oxidation State Ferric Ferric
Spin State LS LS
EPR data g = 2.91, 2.41, 1.50 g = 2.90, 2.41, 1.54
rRaman data 1364 (v4), 1504 (v3), 1640 (v10), no 1480 (v3) 1361 (v4), 1504 (v3), no 1480 (v3)
MCD data 25130 cm−1,24050 cm−1 25280 cm−1, 24300 cm−1

DF = diferric enzyme, SR = semi-reduced enzyme, LS = low-spin, HS = high-spin

a

HS ferrous H-heme results from photolysis of the Fe-Smet bond