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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: J Invest Dermatol. 2018 May 19;138(11):2423–2431. doi: 10.1016/j.jid.2018.04.033

Figure 4. VDR ablation impairs SC-driven epidermal regeneration during wound re-epithelialization.

Figure 4.

(a) The strategy for lineage tracing using Krt14CreERT2RosaTdT. (b) Representative images of initial skin biopsy (0 days) (left panels) and after 30 days of wound healing (middle panels), TdT-labeled SCs migrated to form clones in CON (white triangles, upper panels), but not in cKO (bottom panels). Differentiation was verified by FLG (right two panels) (TdT red; FLG green; DAPI blue). Scale bar = 25 μm (c) Quantification of the TdT-labeled cells in the epidermis by Bioquant (**P < 0.05). (d) Migration of epithelial tongues at 3 days, in which wounding edge is shown by red bolt, and the extent of the epithelial tongue is shown by arrows. Scale bar = 100 μm. (e) Quantitated migration at 3 days (n = 3, P < 0.05). cKO, conditional knockout CON, control; SC, stem cell; TdT, TdTomato; VDR, vitamin D receptor.