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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: Biochemistry. 2019 Nov 4;58(45):4480–4493. doi: 10.1021/acs.biochem.9b00653

Figure 6. Metal ion dissociation rates for zinc(II) and iron(II)-constituted S39E HDAC8.

Figure 6.

Initial rates for Zn(II)-constituted S39E HDAC8 (filled blue circles) and Fe(II)-constituted S39E HDAC8 (filled red circles) deacetylation activity as a function of time after addition of 1 mM EDTA, as measured using the Fluor-de-Lys assay. The fraction activity is determined by dividing the activity in EDTA by the activity of HDAC8 incubated in the absence of EDTA. The koff values were calculated by fitting an exponential decay equation to data from replicates on different days using global regression analysis (GraphPad Prism).