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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: J Immunol. 2018 May 23;201(1):230–242. doi: 10.4049/jimmunol.1701216

Figure 1. Expression of TLR1 prevents mucosal-associated bacteria and their translocation to the periphery.

Figure 1

Colons from naïve 1KO mice were compared to naïve WT colons. (A) Representative 40X confocal images from Carnoy’s fixed sections of the distal colon of WT and 1KO mice hybridized with a probe against eubacterial 16S rRNA (red) and counter stained with DAPI (blue). Image on far right is a fluorescent microscope image of 1KO with using the 16S FISH probe (red), DAPI (blue) and DBA lectin (green). (B) Fold change of 16S DNA expression from 1KO luminal colonic contents or colonic mucosal scrapings normalized to WT. (C) Relative fluorescent units in the serum of WT and 1KO mice 1 hour post-intrarectal administration of FITC-Dextran. Each dot represents an individual mouse. (D) Endotoxin units from serum of WT and 1KO mice as determined using LAL test. Each dot represents an individual mouse. (E) Representative bacterial plates from livers of 1KO and WT mice. (F) CFU per gram of liver cultured anaerobically on TSA plates. Each dot represents an individual mouse. (Data is represented as the mean ± s.e.m from 2–3independent experiments. A, n= 3 mice per group; B–F, n= 7–10 mice per group; *, p<0.05, **, p<0.01. Student’s unpaired t-test.