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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: J Immunol. 2018 May 23;201(1):230–242. doi: 10.4049/jimmunol.1701216

Figure 7. Loss of TLR1 exacerbates DSS-induced cytokine production and prevents colonic repair.

Figure 7

WT and 1KO littermates were administered drinking water containing 2.5% DSS for 7 days to induce epithelial injury followed by a 7-day recovery period in which they were returned to normal drinking water. (A) Percent change in weight, (B) survival, (C) colon length at days 7 and 14 (D), fecal occult score. At days 7 and 14 colons were fixed and stained for (E) H&E and (F) histological score was given after analysis by a gastroenterologist. (G) Concentration of IL-1ß and IL-23 found in whole colon homogenates. (H) IL-17, IL-22 and IFN-γ levels from the supernatants of colonic explant cultures derived from naive mice or mice treated with DSS for 10 days. (I) Mean number of cells in the colonic LP determined by flow cytometry for indicated cell type gated as described in Fig 1. legend. A–D, data is expressed as mean ± s.e.m from 3 independent experiments, n=8–10 mice per group. E, the mean of histological scores from 2 independent experiments, n=3 mice per group. F, representative microscopy images of 3 mice per group. G–I, data is expressed as mean ± s.e.m from two independent experiments with n=6–8 mice per group. A, Wilcoxon log rank test; B, Kaplan Meyer; C, E, G, H, I Students t-test; D, Two-way ANOVA. *, p<0.05, **, p<0.01. ***, p <0.001.