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. 2019 Sep 11;10(6):1210–1227. doi: 10.1002/jcsm.12459

Figure 6.

Figure 6

Blocking SIRPα in muscle facilitates inter‐organ communication and prevents cachexia in CKD. At 8–12 weeks after subtotal nephrectomy (A–G: n = 4–6 mice/group): (A) blood urea nitrogen (BUN), (B) grip strength, (C) glucose tolerance, and (D) insulin levels were measured by ELISA. (E) Organ harvest weight normalize to tibia length (TL) was measured. (F) Protein lysates of gastrocnemius (Gas) skeletal muscle were immunoblotted (top panel) to detect SIRPα, pAkt (Ser473), and GAPDH; and relative densities were measured (bottom panel). (G) Inguinal white adipose tissue (iWAT) was immunoblotted (top panel) to detect SIRPα, pCREB, pAkt (Ser473), UCP1, phosphorylated protein kinase A [pPKA (RRXS/T)], and relative densities obtained to GAPDH are shown (bottom panel). (H) ELISA was performed to detect circulating serum levels of SIRPα. Values are expressed as mean ± SEM; *P < 0.05, sham vs. CKD and #P < 0.05, SIRPαfl/fl vs. mSIRPα−/−.