Figure 7.

Blocking SIRPα in adipose tissue does not lead to significant inter‐organ communication or prevention of cachexia (A–G: n = 4–6 mice/group). (A) Glucose tolerance, (B) insulin levels, (C) serum cholesterol, and (D) grip strength were measured. (E) Organ harvest weight was measured. (F) Protein lysates of inguinal and epididymal white adipose tissue (iWAT/eWAT) were immunoblotted (left panel) to detect phosphorylated protein kinase A [pPKA (RRXS/T)], SIRPα, pAKT (Ser473), pCREB, UCP1, and GAPDH. Protein lysates of gastrocnemius skeletal muscle were immunoblotted (left panel) to detect either SIRPα or pAkt (Ser473), and relative densities obtained to GAPDH are shown (right panel). (G) Serum SIRPα was measured by immunoblot. Values are expressed as mean ± SEM; *P < 0.05, sham vs. CKD and #P < 0.05, SIRPα^fl/fl vs. AD‐SIRPα^−/−.