Table 2:
Comparison of quantitation of mRNA, protein, and enzyme activity identified for the TCA enzymes measured and Mmut in the Mut-ko/ki and the Ctl mouse liver samples. In general, protein levels appear to better correlate to enzyme activity than mRNA amounts. * p<0.05, **p<0.01
| Enzyme | Mut-ki/ko mRNA (Avg log2) levels | Ki/wt mRNA (Avg log2) levels | Mut-ko/ki ratio with Ki/wt mRNA ratio | Mut-ki/ko Protein (Amt/Amt Vdac1) | Ki/wt Protein (Amt/Amt Vdac1) | Mut-ko/ki ratio with Ki/wt Protein ratio | Mut-ko/ki Enzyme (nmol* s−1 * mg1) | Ki/wt Enzyme nmol* s−1 * mg−1) | Mut-ko/ki ratio with Ki/wt Enzyme ratio |
|---|---|---|---|---|---|---|---|---|---|
| CS | 10.29±0.59 | 10.54±0.47 | 0.97 | 0.96±0.18 | 1.0 +0.12 | 0.95 | 68 | 62 | 1.1 |
| IDH2(3) (Idh2) | 10.36±0.4 | 9.51±0.15 | 1.09 | 0.75±0.15 | 1.02±0.22 | 0.74 | 43 | 50 | 0.87 |
| OGDHc (Ogdh) | 9.14±0.76 | 8.91±0.15 | 1.03 | 0.58* | 1.01 | 0.57 | 1.2* | 1.7 | 0.67 |
| SCS (Suclg2) | 9.82±0.42 | 9.91±0.66 | 0.99 | 0.95 | 1.01 | 0.93 | 140 | 130 | 1.1 |
| SDH (Sdha) | 7.22±0.82 | 7.77±0.01 | 0.92 | 0.7 | 1.04 | 0.67 | 15 | 16 | 0.91 |
| Fh | 11.27±0.32 | 10.22±0.03 | 1.1 | 0.57* | 1 | 0.57 | 7.8 | 12 | 0.67 |
| MDH2 | 7.81±0.76 | 9.01±0.97 | 0.86 | 0.66* | 1 | 0.66 | 190 | 250 | 0.75 |
| Mut (via SS-SDH) | 6.59±0.37 | 9.48±1.37 | 0.69 | 0.49** | 1 | 0.49 | 0.293 | 1.033 | 0.28 |