Fig. 1.
Systemic inflammation breaks tolerance to a transgene product. (A) A schematic of the experimental design detailing i.v. administration of AAV8 expressing hOTCco-SIINFEKL 40 μg CpG ODN for three days following adoptive transfer. Serum (B) ALT and (C) AST levels were monitored every seven days following vector delivery. (D) Genome copy and (E) transgene mRNA levels from liver were determined using qPCR after the study terminated. These experiments were independently performed three times; here we show representative results (N = 3). Statistical analysis by ANOVA with a Bonferroni posttest to vector only control. *P < 0.05, **P < 0.001, ***P < 0.0001. Error Bars = SEM.