Effect of T-cell population on immune response. Mice received i.v. injections of AAV8 expressing hOTCco-SIINFEKL; 28 days after vector delivery, we transferred OT-1 CD8+ T cells to recipient mice concurrent with an administration of 40 μg of CpG ODN. Serum (A) AST, (B) ALT, and (C) bilirubin levels were monitored every seven days following vector delivery. (D) Genome copy and (E) transgene mRNA levels from liver were determined by qPCR following the termination of the study. N = 3. Statistical analysis by ANOVA with a Bonferroni posttest to vector only control. *P < 0.05, **P < 0.001, ***P < 0.0001, ****P < 0.00001. Error Bars = SEM.