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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Cell Immunol. 2019 Oct 26;346:103997. doi: 10.1016/j.cellimm.2019.103997

Fig. 5.

Fig. 5.

Transgene-specific T-cell infiltration. Mice were given AAV8 hOTCco-SIINFEKL; 14 days later, we administered the CpG ODN and CFSE-labeled OT-1 CD8+ T cells. Mice were sacrificed seven days following CD8+ T-cell transfer, and we isolated lymphocytes from the liver and spleen. Lymphocytes isolated from the (A) spleen and (C) liver by forward and side scatter were stained for CD8. (B) Spleen-and (D) liver-isolated CD8+ T cells were stained with SIINFEKL tetramer. (E) We analyzed tetramer-positive liver lymphocytes for CFSE retention. (F) Here we show a representative single parameter histogram for analysis of CFSE retention in cell populations with and without CpG ODN treatment. N = 4. *P < 0.05, **P < 0.001. Error Bars = SEM.