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. 2019 Dec 10;14(12):e0214351. doi: 10.1371/journal.pone.0214351

Fig 7. Treatment experiment: TUNEL staining.

Fig 7

A: TUNEL staining was performed to detect apoptotic cells at 10 weeks after surgery. DAPI/TUNEL double staining is shown in each group (DAPI staining, TUNEL staining, DAPI/TUNEL staining, Bar = 100 μm). The vehicle group showed the highest number of TUNEL-positive cells. B: The number of TUNEL-positive cells was counted in each group. The number of TUNEL-positive cells in the vehicle group was significantly higher than in the other two groups (*P < 0.0001: one-way ANOVA), with no significant difference between the sham and high-dose EPO groups. C: The percentage of TUNEL-positive cells in each group. The percentage in the vehicle group was significantly higher than in the other two groups (*P < 0.0001: one-way ANOVA).