Table 3. Cleavage data averaged from cleavage assays products ran in triplicate (n = 3) and analyzed by densitometry.

Guide ID	Template ID	Avg.Uncleaved Template (%)	Avg. Cleaved Template (%)	Standard Error of the mean (+/-)
1	Off Target 1	93.47	6.53	3.70
1	Off Target 2	97.71	2.29	0.51
1	Native	24.28	75.72	6.03
2	Off Target 1	93.50	6.50	3.08
2	Off Target 2	97.43	2.57	0.66
2	Native	82.14	17.86	3.14
3	Off Target 1	91.92	8.08	3.55
3	Off Target 2	96.54	3.46	0.32
3	Native	89.25	10.75	1.69
Pos	Pos	9.46	90.54	5.75
Neg	Neg	100.00	0.00	0.00
4	Off Target 1	79.32	20.68	9.15
4	Off Target 2	89.93	10.07	6.91
4	Native	36.53	63.47	15.24
5	Off Target 1	76.03	23.97	7.06
5	Off Target 2	90.13	9.87	5.35
5	Native	23.88	76.12	10.20
6	Off Target 1	83.81	16.19	7.19
6	Off Target 2	88.61	11.39	5.25
6	Native	31.02	68.98	10.04
Pos	Pos	4.87	95.13	2.40
Neg	Neg	95.50	4.50	4.30
7	Off Target 1	16.26	83.74	1.63
7	Off Target 2	82.43	17.57	2.57
7	Native	7.72	92.28	1.94
8	Off Target 1	28.31	71.69	2.01
8	Off Target 2	83.89	16.11	0.90
8	Native	8.82	91.18	1.60
9	Off Target 1	63.39	36.61	2.60
9	Off Target 2	92.99	7.01	0.86
9	Native	56.08	43.92	0.66
10	Off Target 1	41.43	58.57	7.19
10	Off Target 2	89.54	10.46	2.43
10	Native	17.94	82.06	1.08
Pos	Pos	3.06	96.94	0.66
Neg	Neg	99.88	0.12	0.34

Standard error is shown (+/-).