Fig 1. Bacterial load in lung and spleen of binge-drinking mice intranasally infected with decreasing doses of B. thailandensis.

Colony forming units (CFUs) per lung (A). Colony forming units (CFUs) per spleen (B). C57BL/6 mice were administered alcohol (4.4 g/kg) or PBS intraperitoneally (i.p.) and 0.5 h later mice were inoculated intranasally with B. thailandensis at doses of (3 x 10⁵, 5 x 10⁴, 8 x 10³, or 500). Tissues were collected 24 h post infection and bacterial tissue burden was determined (CFU/Tissue). Each bar represents the mean of each group inoculated with a respective dose with SD, N = 6 per group. Average fold increase comparison of lung and spleen tissue (C). Fold increase is based on initial bacterial dose and mean final bacterial burden with SD. ND = Not Detected; no bacteria was cultured on any LB media plate. (Control) indicates infected mice (3 x 10⁵ CFU) not administered alcohol. Horizontal lines and asterisks (*) represent statistical comparison of (10⁵ dose) and subsequent lower doses by Student’s t-test with Welch’s correction. ****, p ≤ 0.0001.