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. 2019 Dec 10;14(12):e0218147. doi: 10.1371/journal.pone.0218147

Fig 2. Bacterial load in lung and spleen of mice intranasally infected with B. thailandensis and administered different alcohol doses.

Fig 2

Colony forming units (CFUs) per lung (A). Colony forming units (CFUs) per spleen (B). C57BL/6 mice were administered alcohol at doses of (4.4, 3, 2, 1 g/kg) or PBS intraperitoneally (i.p.) and 0.5 h later mice were inoculated intranasally with B. thailandensis. Dashed line represents the inoculating bacterial dose of (3 x 105) CFUs. Tissues were collected 24 h post infection and bacterial tissue burden was determined (CFU/Tissue). Each bar represents the mean of each group administered a respective alcohol dose with SD, N = 6 per group. Measured BAC (%) indicated with () and line represents average BAC measured from blood collected 0.5 h prior to infection from each group (0.254, 0.152, 0.0265, 0.00397%) or PBS respectively. ND = Not Detected; no bacteria was cultured on any LB media plate. Horizontal lines and asterisks (*) represent statistical comparison of (4.4 g/kg dose) and subsequent lower alcohol doses by Unpaired Student’s t-test. **, p ≤ 0.01, ****, p ≤ 0.0001.