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. 2019 Dec 10;14(12):e0218147. doi: 10.1371/journal.pone.0218147

Fig 6. B. thailandensis invasion and survival in non-phagocytic lung and intestinal cells with and without alcohol treatment.

Fig 6

Murine (Eph4, LET-1), intestinal (Mode K), or human (A549) lung epithelial cells were grown to confluency in cell culture media and co-cultured with B. thailandensis (MOI 1:10) with 0.0% or 0.2% v/v alcohol. ALC (Prior to infect) indicates cells treated with alcohol for 3 h prior to infection. ALC (During infect) indicates cells treated with alcohol at the time of infection. All cells were co-cultured for 3 h post alcohol treatment. Extracellular bacteria were removed by washes X4 and antibiotic treatment for 2 h. Cells were lysed, and viable bacteria recovered. Asterisks (*) represent statistical comparisons between ALC (Prior to infect) or (During infect) treatment and (Control, Non-Alcohol) determined by two-way ANOVA and Dunnett’s multiple comparisons test. Bars represent average CFU with SD. ***, p ≤ 0.001, ****, p ≤ 0.0001. Group comparison to Mode K cells determined by two-way ANOVA and Tukey’s multiple comparison test, **** p ≤ 0.0001, N = 3.