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. 2019 Dec 10;14(12):e0226163. doi: 10.1371/journal.pone.0226163

Fig 4. PTH enhances the proliferation and physiological function of endothelial cells in vitro.

Fig 4

(A) HUVECs were treated with PTH or saline for 48 h (PTH treated 1 h every 24 h), and a CCK-8 assay was performed to determine the endothelial cell proliferative activity. (B) Western blot analysis for the expression of the VEGF and COX-2 protein levels. (C) qPCR for mRNA level analysis related to the function of endothelial cells after PTH treatment compared to that of the control. (D) The formation of tube-like networks was examined at 1.5 h, 3 h and 6 h by a tube formation assay. Representative micrographs obtained at 10× (D, scale bar, 50 μm). Student’s t test for two groups comparisons was performed; **P < 0.01, and ***P < 0.001.