Skip to main content
. 2015 Jun 18;47(2):701–709. doi: 10.3892/ijo.2015.3057

Figure 2.

Figure 2

Rg3 induces human melanoma cell apoptosis. (A) A375P and C8161 cells were double stained with Annexin V/PI and analyzed by FACS analysis after 24-h treatment with Rg3 (0, 25, 50, 75 and 100 μg/ml). The percentage of Annexin V+/PI− (early apoptotic cells, lower right), Annexin V+/PI+ (late apoptotic cells, upper right), Annexin V−/PI− (viable cells, lower left) and Annexin V−/PI+ (necrotic cells, upper left), cells is shown. (B) A375P and C8161 cells were treated with Rg3 (0, 25, 50, 75 and 100 μg/ml) for 24 h, the expression levels of PARP, caspase-8, 9, 3, Bcl-2, survivin and Bax were analyzed by western blot analysis.