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. 2015 Jun 18;47(2):701–709. doi: 10.3892/ijo.2015.3057

Figure 3.

Figure 3

Rg3 inhibits the activation of NF-κB signaling pathway. (A) Analysis of phosphorylation and non-phosphorylation levels of NF-κB/p65, IKKα/β and IκBα levels by western blot analysis in A375P and C8161 cells after cells were treated with Rg3 (75 μg/ml) for 24 h. (B) Cells were treated with 75 μg/ml of Rg3 for 24 h. The nuclear levels of the proteins were detected by western blot assay. Lamin-A was used as nuclear loading control. (C) A375P and C8161 cells were preincubated with Rg3 (75 μg/ml) for 24 h. EMSA was performed in nuclear extract for DNA binding of activated NF-κB assay. (D) Cells were transfected with NF-κB luciferase reporter for 24 h, and then preincubated with Rg3 (75 μg/ml) for 24 h. Luciferase activity was measured in the cell lysate. Data are expressed as means ± SD. *P<0.05; **P<0.01.