Skip to main content
. 2015 Jun 18;47(2):701–709. doi: 10.3892/ijo.2015.3057

Figure 4.

Figure 4

Rg3 decreases FUT4 expression and inhibits NF-κB binging to FUT4 promoter. FUT4 was detected by (A) western blotting and (B) real-time PCR in A375P and C8161 cells after treatment with Rg3 (75 μg/ml) for 24 h. p65 and FUT4 expression by (C) real-time PCR and (D) western blotting in A375P and C8161 cells. Control, untransfected cells; Mock, cells transfected with vector; p65 siRNA, cells transfected with p65 siRNA; cells were exposed to Bay 11-7082 (10 μM) for 24 h. (E) After treatment with Rg3 (75 μg/ml) for 24 h, chromatin in the treated cells was immunoprecipitated with antibodies to p65 and the FUT4 promoter region in the precipitated chromatin was amplified by PCR. Data are presented as the mean ± SD of three independent experiments. *P<0.05; **P<0.01.