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. 2015 Jun 11;47(2):592–600. doi: 10.3892/ijo.2015.3044

Figure 3.

Figure 3

Modulation of apoptotic and cell cycle regulatory proteins in Ramos cells treated with HCS. (A) Ramos cells were treated with HCS (0, 12.5, 25 and 50 μl/ml) for 24 h, followed by western blot analysis to detect caspase-3, cdk2, cdk4, p21CIP1, survivin, Rb and pRb proteins. (B) Cells were treated with the indicated concentrations of HCS with or without the caspase inhibitor, ZDEVD, for 24 h and immunoblotted with the caspase-3 antibodies. (C) The effect of ZDEVD on HCS induced apoptotic cell death by Annexin V staining. ZDEVD markedly blocked the early phase of apoptosis induced by HCS while moderately modulated the late phase of apoptotic or necrotic cells (top, bar graph of early phase apoptosis; bottom, histogram). Each experiment was performed in duplicate and repeated twice independently. Data are presented as mean ± SD.