Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 27;44(5):1661–1668. doi: 10.3892/ijo.2014.2313

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

PMC Copyright notice

Figure 1. — Spautin-1 inhibits IM-induced autophagy in K562 cells. After treatment with or without IM (250 nM) for 12 h, spautin-1 (10 μM) or DMSO (0.1%) was added to K562 medium for further 36 h. (A) Autophagy was denoted by the switch of LC3I to LC3II, which was detected by western blotting (WB). (B) Other autophagy factors such as Beclin-1, ULK1, ATG 5 and ATG 7 were also detected by WB. (C) The bar chart demonstrates the ratio of LC3II and Beclin-1 proteins to tubulin by densitometry. Data are expressed as mean ± SD (^#,*P≤0.05 between groups).

Figure 1. — Spautin-1 inhibits IM-induced autophagy in K562 cells. After treatment with or without IM (250 nM) for 12 h, spautin-1 (10 μM) or DMSO (0.1%) was added to K562 medium for further 36 h. (A) Autophagy was denoted by the switch of LC3I to LC3II, which was detected by western blotting (WB). (B) Other autophagy factors such as Beclin-1, ULK1, ATG 5 and ATG 7 were also detected by WB. (C) The bar chart demonstrates the ratio of LC3II and Beclin-1 proteins to tubulin by densitometry. Data are expressed as mean ± SD (^#,*P≤0.05 between groups).