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Confirmation of exosomal vesicles isolated from PCa cell culture supernatant. Western blot analysis demonstrated significant enrichment of exosomal marker proteins in the pellet of supernatant from 4 kinds of cells after ultracentrifugation (A). Electron micrograph (EM) of isolated exosomes in the supernatant from PC3 (B) and DU145 (C). The bar, 200 nm.
