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. 2019 Dec 4;9:413. doi: 10.3389/fcimb.2019.00413

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Copyright © 2019 Hernández-de-los-Ríos, Murillo-Leon, Mantilla-Muriel, Arenas, Vargas-Montes, Cardona, de-la-Torre, Sepúlveda-Arias and Gómez-Marín.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine secretion by PBMCs infected with the RH strain of T. gondii. The concentration of IFN-γ (A), IL-10 (B), and IL-1β (C) were determined by ELISA in supernatants at 24 h post-infection (h.p.i.). A correlation analysis on RH, RHΔrop16 and RHΔrop18 strains for each cytokine was performed. The x axis indicates the concentration of IFN-γ (D), IL-10 (E), and IL-1β (F) for RH infections. The y axis indicates the same cytokine as in x axes for RHΔrop16 (black circles) or RHΔrop18 (red squares) T. gondii infected cells. Each point represents the concentration of cytokines of a single patient. Results (A–C) are represented as mean ± SD; *p < 0.05, Mann-Whitney U test and the correlations were determined by Spearman's correlation tests. OT, Ocular toxoplasmosis; Asym, Chronic asymptomatic infection; Neg, T. gondii seronegative individuals.