Figure 3.

Antigen presentation machinery is not impaired in MVA-infected cells. OVA-expressing E.G7-OVA cells were incubated with ice-cold acid stripping buffer (131 mM sodium citrate, 66 mM sodium phosphate, and 1% BSA pH 3) for 2 min to allow for removal of surface MHC molecules from the cell surface. After washing with medium, stripped cells were infected with MVA-GFP under the control of the early/late promoter P7.5 (MVA-Pe/l-GFP) at MOI 10 or mock for the indicated hours. After respective incubation time, cells were washed and surface staining was performed with anti-H-2K^b Ab (A) or anti-SIINFEKL/K^b (B) after viability dye staining determining the de novo synthesis of peptide-loaded MHC class I molecules. (C) MFI of GFP indicated MVA-infected cells. All data are means and SEM (n ≥ 3) from three independent experiments.