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. 2019 Dec 4;10:2806. doi: 10.3389/fmicb.2019.02806

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Copyright © 2019 Morguette, Bigotto, Varella, Andriani, Spoladori, Pereira, de Andrade, Lancheros, Nakamura, Arakawa, Bruschi, Tomaz, Lonni, Kerbauy, Tavares, Yamauchi and Yamada-Ogatta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal laser scanning microscopy images of mature biofilm of Streptococcus agalactiae ATCC 13813 untreated control (A1,A2) and treated with copaiba oil (B1,B2), CARB-BF (C1,C2) and CARB-CO 1.0 (D1,D2). Biofilms were formed on CELLview^TM cell culture dish with glass bottom during 24 h at 37°C before the treatments. Cells were stained with SYTO 9^® (green-fluorescent) and propidium iodide (red-fluorescent). Panoramic view of biofilm (A1–D1). Three-dimensional biofilm reconstitution (A2–D2).