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. 2019 Dec 4;10:2793. doi: 10.3389/fimmu.2019.02793

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Copyright © 2019 Raman, Mejías-Pérez, Gomez, García-Arriaza, Perdiguero, Vijayan, Pérez-Ruiz, Cuervo, Santiago, Sorzano, Sánchez-Corzo, Moog, Burger, Schorcht, Sanders, Carrascosa and Esteban.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production and characterization of recombinant proteins. (A) Scheme of production and characterization of the GP120C14K protein. (B) Size exclusion chromatograms of GP120C (left) and GP120C14K (right) purified proteins. (C) Electrophoresis of GP120C (lane 1: 1,000 ng, lane 2: 500 ng) and GP120C14K (lane 3: 500 ng, lane 4: 1,000 ng) proteins under native/non-reducing conditions (4–15% gel, silver stained); Lane M: Native marker. (D) Elution profile (elution volume against absorbance) of GP120C14K obtained through Size Exclusion Chromatography Coupled Multi-Angle Light Scattering (SEC-MALLS) technique.