Skip to main content
. 2019 Dec 4;10:2793. doi: 10.3389/fimmu.2019.02793

Figure 5.

Figure 5

Time-course expression of HIV-1 Env SOSIP proteins expressed from MVA vectors by Western-blot analysis. (A) Monolayers of DF-1 cells were infected at 5 PFU/cell with MVA-WT, MVA-ZM197 SOSIP, or MVA-AMC011 SOSIP viruses. At the indicated times post-infection, infected cells were collected, cells extracts fractionated by 10% SDS-PAGE and analyzed by Western-blot using rabbit polyclonal anti-GP120 antibody to evaluate the expression of the SOSIP proteins in cellular pellet (P) and supernatant (SN). (B) bNAbs binding profile to Env antigens by flow cytometry. HeLa cells infected with MVA-GP120C14K, MVA-AMC011 SOSIP, MVA-ZM197 SOSIP, or MVA-GPN viruses were processed for flow cytometry as described under Materials and Methods using 10 μg/ml of the indicated primary human IgG anti-Env bNAb. Samples were acquired in a flow cytometer and geometric Mean Fluorescence Intensity (gMFI) values on the “live cells” gate were used to analyse the results.