Figure 9.

Anti-Env total IgG antibody levels and HIV-1 neutralization capacity in immunized rabbits. (A) Immunization schedule and immunization groups (n = 4) for the evaluation of the binding antibodies and the neutralization capacity of GP120C14K protein compared with the SOSIP constructs AMC011 and ZM197. (B) Anti-Env antibody levels (total IgG) in the serum of immunized rabbits at weeks 2, 6, 10, and 14 measured by ELISA. End-point titers of antibody responses obtained for each group were calculated from the reactivity shown against the corresponding protein and referred as the last dilution that provided an optical density value (at 450 nm) that is more than three times the value obtained at the same dilution by the naïve serum. (C) Neutralization titers (IC50) for the different immunization groups at weeks 0, 6, 10, and 14 against HIV-1 tier 1 (MW965.26 and SF162) and tier 2 (BG505 and sC22) viruses.