Figure 4.

The effect of CcpA on the formation of biofilm. SK36, ΔCcpA, and CcpA+ strains cultures were inoculated into microtiter plates and anaerobically grown as static cultures for 48 h at 37°C to form biofilms. (A) Representative of microtiter plate wells from each experiment showing the respective biofilm formation of each S. sanguinis strain. (B) Quantitative analysis of biofilm production measuring at 570 nm using a microplate reader. The data presented are averages and standard deviations of three independent experiments with similar results, triplicate in each experiment. *Indicates the significant difference at P < 0.05 compared to the SK36.