Table 1.
Shoot | Root | |||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
High N | High N +OP | Low N | Low N +OP | High N | High N +OP | Low N | Low N +OP | |||||||||||||||||||||||||
Carbohydrates (µmol g -1 FW) | ||||||||||||||||||||||||||||||||
Glucose | 3.33 | ± | 1.10 | c | 4.22 | ± | 0.53 | c | 5.30 | ± | 0.23 | b | 6.71 | ± | 0.70 | a | 5.05 | ± | 0.37 | bc | 4.83 | ± | 0.22 | c | 6.40 | ± | 1.25 | b | 8.99 | ± | 0.91 | a |
Fructose | 2.53 | ± | 0.19 | a | 1.76 | ± | 0.02 | b | 1.93 | ± | 0.63 | ab | 2.76 | ± | 0.94 | a | 2.35 | ± | 0.63 | a | 0.66 | ± | 0.48 | b | 2.20 | ± | 0.66 | ab | 1.35 | ± | 0.25 | b |
Starch | 5.57 | ± | 1.52 | c | 11.03 | ± | 2.98 | b | 10.06 | ± | 3.52 | bc | 38.87 | ± | 8.05 | a | 5.67 | ± | 1.13 | c | 10.49 | ± | 3.52 | b | 13.17 | ± | 7.47 | bc | 43.90 | ± | 9.00 | a |
Sucrose | 1.12 | ± | 0.10 | c | 0.82 | ± | 0.03 | d | 1.44 | ± | 0.14 | b | 2.29 | ± | 0.23 | a | 1.80 | ± | 0.48 | a | 0.84 | ± | 0.22 | b | 1.17 | ± | 0.13 | b | 2.30 | ± | 0.62 | a |
Soluble proteins (mg g-1 FW) | ||||||||||||||||||||||||||||||||
Soluble proteins | 6.35 | ± | 1.60 | a | 4.61 | ± | 0.37 | a | 4.49 | ± | 0.18 | a | 3.58 | ± | 0.49 | b | 0.65 | ± | 0.38 | c | 1.52 | ± | 0.41 | b | 1.63 | ± | 0.25 | b | 3.22 | ± | 0.67 | a |
Amino acids (µmol g -1 FW) | ||||||||||||||||||||||||||||||||
Total amino acid | 30.85 | ± | 5.99 | a | 21.45 | ± | 2.12 | b | 13.20 | ± | 4.10 | c | 36.44 | ± | 8.57 | a | 31.03 | ± | 6.96 | b | 44.97 | ± | 6.76 | a | 10.88 | ± | 0.93 | d | 19.33 | ± | 3.35 | c |
Alanine | 1.31 | ± | 0.26 | b | 0.92 | ± | 0.08 | c | 2.07 | ± | 0.70 | b | 7.54 | ± | 1.84 | a | 0.92 | ± | 0.24 | d | 2.94 | ± | 0.42 | b | 1.76 | ± | 0.30 | c | 7.48 | ± | 1.61 | a |
Asparagine | 21.88 | ± | 4.35 | a | 14.57 | ± | 1.58 | b | 2.84 | ± | 0.92 | d | 7.97 | ± | 1.77 | c | 17.19 | ± | 4.08 | a | 24.07 | ± | 3.52 | a | 1.29 | ± | 0.17 | b | 1.54 | ± | 0.32 | b |
Aspartate | 0.58 | ± | 0.11 | b | 0.43 | ± | 0.03 | c | 0.57 | ± | 0.17 | bc | 1.08 | ± | 0.24 | a | 1.42 | ± | 0.36 | ab | 1.66 | ± | 0.38 | a | 1.00 | ± | 0.10 | b | 1.20 | ± | 0.15 | ab |
BCAAs | 0.38 | ± | 0.14 | b | 0.29 | ± | 0.02 | b | 0.34 | ± | 0.05 | b | 0.79 | ± | 0.12 | a | 0.75 | ± | 0.13 | a | 0.87 | ± | 0.09 | a | 0.76 | ± | 0.09 | a | 0.79 | ± | 0.12 | a |
GABA | 0.03 | ± | 0.01 | bc | 0.03 | ± | 0.00 | c | 0.04 | ± | 0.01 | b | 0.18 | ± | 0.05 | a | 0.08 | ± | 0.02 | b | 0.12 | ± | 0.03 | ab | 0.04 | ± | 0.01 | c | 0.18 | ± | 0.04 | a |
Glutamate | 1.65 | ± | 0.32 | b | 1.12 | ± | 0.07 | c | 1.72 | ± | 0.57 | bc | 3.57 | ± | 0.75 | a | 2.65 | ± | 0.42 | ab | 2.88 | ± | 0.59 | ab | 2.10 | ± | 0.24 | b | 3.22 | ± | 0.41 | a |
Glutamine | 0.96 | ± | 0.20 | b | 0.30 | ± | 0.02 | c | 0.69 | ± | 0.23 | b | 2.24 | ± | 0.73 | a | 3.92 | ± | 0.98 | b | 7.81 | ± | 1.08 | a | 1.24 | ± | 0.10 | c | 1.38 | ± | 0.24 | c |
Glycine | 1.45 | ± | 0.23 | bc | 1.41 | ± | 0.09 | c | 2.15 | ± | 0.59 | b | 4.26 | ± | 1.03 | a | 0.82 | ± | 0.09 | a | 0.53 | ± | 0.06 | b | 0.23 | ± | 0.03 | c | 0.43 | ± | 0.06 | b |
Minor amino acids | 0.80 | ± | 0.22 | b | 0.62 | ± | 0.05 | b | 0.72 | ± | 0.15 | b | 2.35 | ± | 0.41 | a | 1.56 | ± | 0.31 | ab | 2.05 | ± | 0.29 | a | 1.45 | ± | 0.16 | b | 1.48 | ± | 0.25 | b |
Monoethanolamine | 0.27 | ± | 0.06 | b | 0.20 | ± | 0.02 | b | 0.24 | ± | 0.09 | b | 0.60 | ± | 0.15 | a | 0.46 | ± | 0.11 | a | 0.43 | ± | 0.10 | a | 0.30 | ± | 0.01 | b | 0.38 | ± | 0.04 | a |
Ornithine | 0.24 | ± | 0.05 | a | 0.17 | ± | 0.08 | abc | 0.11 | ± | 0.01 | c | 0.15 | ± | 0.01 | b | 0.90 | ± | 0.15 | a | 0.90 | ± | 0.08 | a | 0.40 | ± | 0.07 | b | 0.32 | ± | 0.03 | b |
Proline | 0.30 | ± | 0.06 | ab | 0.34 | ± | 0.03 | ab | 0.30 | ± | 0.01 | b | 0.38 | ± | 0.04 | a | 0.27 | ± | 0.01 | a | 0.33 | ± | 0.06 | a | 0.22 | ± | 0.01 | b | 0.35 | ± | 0.08 | a |
Serine | 1.29 | ± | 0.25 | b | 1.27 | ± | 0.11 | b | 1.69 | ± | 0.63 | b | 6.21 | ± | 1.65 | a | 0.71 | ± | 0.17 | b | 1.11 | ± | 0.20 | a | 0.71 | ± | 0.06 | b | 1.19 | ± | 0.22 | a |
Threonine | 0.09 | ± | 0.02 | b | 0.07 | ± | 0.02 | bc | 0.05 | ± | 0.02 | c | 0.49 | ± | 0.15 | a | 0.15 | ± | 0.04 | ab | 0.22 | ± | 0.04 | a | 0.16 | ± | 0.02 | b | 0.18 | ± | 0.04 | ab |
Ions and hydrogen peroxide (µmol g -1 FW) | ||||||||||||||||||||||||||||||||
Acetate | 3.48 | ± | 0.30 | a | 3.10 | ± | 0.37 | a | 3.27 | ± | 0.72 | a | 1.95 | ± | 0.18 | b | 6.05 | ± | 0.33 | a | 5.76 | ± | 0.68 | a | 4.41 | ± | 0.62 | b | 4.32 | ± | 0.20 | b |
Chloride | 62.51 | ± | 2.95 | a | 52.53 | ± | 2.11 | b | 72.69 | ± | 16.06 | a | 48.63 | ± | 1.59 | c | 32.08 | ± | 3.54 | b | 29.27 | ± | 1.62 | b | 41.95 | ± | 4.41 | a | 42.83 | ± | 6.09 | a |
Malate | 2.32 | ± | 0.12 | c | 1.64 | ± | 0.07 | d | 3.85 | ± | 0.93 | a | 2.73 | ± | 0.05 | b | 1.05 | ± | 0.10 | c | 0.64 | ± | 0.11 | d | 2.09 | ± | 0.25 | b | 2.74 | ± | 0.18 | a |
Nitrate | 36.92 | ± | 1.56 | b | 41.51 | ± | 1.94 | a | 35.34 | ± | 3.48 | b | 35.19 | ± | 0.74 | b | 32.76 | ± | 0.72 | a | 24.18 | ± | 0.78 | b | 21.93 | ± | 1.70 | b | 17.38 | ± | 1.00 | c |
Oxalacetate | 2.16 | ± | 0.10 | a | 1.97 | ± | 0.25 | a | 0.93 | ± | 0.14 | c | 1.21 | ± | 0.12 | b | 4.65 | ± | 0.42 | a | 3.78 | ± | 0.23 | b | 3.96 | ± | 0.64 | ab | 3.57 | ± | 0.13 | b |
Sulphate | 1.82 | ± | 0.08 | b | 2.02 | ± | 0.03 | a | 2.07 | ± | 0.32 | ab | 1.28 | ± | 0.03 | c | 7.84 | ± | 0.60 | c | 7.08 | ± | 0.45 | c | 12.26 | ± | 1.07 | a | 10.57 | ± | 0.16 | b |
Hydrogen peroxide | 3.10 | ± | 0.66 | c | 3.23 | ± | 0.31 | c | 7.38 | ± | 0.64 | a | 4.70 | ± | 0.34 | b | 2.74 | ± | 0.34 | a | 2.70 | ± | 0.57 | a | 2.63 | ± | 0.23 | a | 1.81 | ± | 0.23 | b |
Other compounds | ||||||||||||||||||||||||||||||||
MDA (nmol g-1 FW) | 28.70 | ± | 0.51 | b | 34.32 | ± | 4.93 | a | 24.12 | ± | 3.50 | c | 28.45 | ± | 0.87 | bc | 81.00 | ± | 10.67 | a | 83.91 | ± | 8.57 | a | 51.23 | ± | 5.75 | b | 48.78 | ± | 2.32 | b |
Polyphenols (mgGAE g-1 FW) | 2.34 | ± | 0.19 | a | 1.99 | ± | 0.08 | b | 2.35 | ± | 0.23 | a | 1.74 | ± | 0.13 | c | 1.15 | ± | 0.13 | a | 1.35 | ± | 0.18 | a | 1.34 | ± | 0.21 | a | 1.32 | ± | 0.06 | a |
Means in the same rows with different letters are significantly different (p < 0.05, Student's t-test).