Fig. 8.

Generation of a floxed allele in monkey embryos. a Schematic diagram showing the floxed allele knock-in strategy. Purple triangles indicate PCR primers for RFLP assay. Blue triangles indicate PCR primers for sequencing. Yellow triangles indicate loxP sequences. b RFLP assay using BamHI for detection of the floxed allele. Asterisks indicate positive bands. “NC” indicates negative control, in which wild-type DNAs were used as PCR templates. “PC” indicates positive control, in which knock-in DNAs were used as PCR templates. n = 30 biologically independent samples. c Efficiencies of deletion and floxed allele knock-in. d The inserted sequence in the floxed allele. Source data are provided as a Source Data file.