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. 2019 Dec 10;9:18692. doi: 10.1038/s41598-019-54887-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo analysis of spine density and spine size at juvenile stage. (A) Schematic illustrating in utero electroporation approach targeting layer 2/3 cortical pyramidal neurons and subsequent quantification of dendritic spine density and head size. Scale bar: 50 µm. (B) Representative images showing apical oblique dendrites of neurons at P21 co-expressing either Venus and tdTomato (Control), or mRFP-tagged F-BARx, F-BAR^Δ49, SRGAP2B, or SRGAP2C. Scale bar: 2 µm. (C,D) Quantification of spine density (C) and spine head size (D). Quantifications are shown as frequency distributions. Spine density (segments): n_Control = 26, n_F-BARx = 25, n_F-BAR^Δ49 = 34, n_SRGAP2B = 25, n_SRGAP2C = 25; Spine size (number of spines): n_Control = 1693, n_F-BARx = 2054, n_F-BAR^Δ49 = 3267, n_SRGAP2B = 2900, n_SRGAP2C = 2448; one-way ANOVA with Holm-Sidak’s post hoc multiple comparison test; *p < 0.05, ****p < 0.0001 compared to control.