Fig. 1. Differential effects of atRA on maturation and stemness of Evi1^high LC^LSK_MA9 and Evi1^low LC^CMP_MA9.

a Schematic of experimental design. BM, bone marrow. b Kaplan–Meier plot of mice transplanted with MA9 transduced LSK cells and CMPs (300,000 cells/mouse). n = 3/group; **p < 0.01; log-rank test. c Relative Evi1 mRNA levels in BM LC^LSK_MA9 and LC^CMP_MA9 (left panel) and the corresponding LSCe (right panel). qRT-PCR; n = 3; **p < 0.01; t-test. d–g BM cells (d–f) or BM LCs (Venus⁺ cells; panel g) from terminally ill mice were treated with 1 µM atRA or solvent for 3 days. n = 3; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant; ANOVA followed by Bonferroni's post-hoc test. d Myeloid differentiation. e Proportions of LSCe among LCs. f Proportions of quiescent LSCe (LSCe in G₀). g Colony formation in methyl cellulose, presented as percent of solvent-treated LC^LSK_MA9 in each round of plating.