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. 2019 Dec 10;10(12):944. doi: 10.1038/s41419-019-2172-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic of experimental design. BM, bone marrow; FC, flow cytometry. b In vivo limiting dilution assay to determine the frequency of functional LSCs. Upper: table showing the numbers of responders (defined by the presence of ≥1% Venus⁺ RFP⁺ cells in BM at the time of sacrifice) and the total numbers of evaluable recipients for each cell dose. Data from two independent experiments were combined, and LSC frequencies were calculated by applying the maximum likelihood method using ELDA software. Lower: Plot showing the logarithmized fractions of non-responding recipients of different numbers of LCs. Statistical significance was assessed using the Chi-square test.