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. 2019 Dec 10;9:18766. doi: 10.1038/s41598-019-55210-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PufM protein Maximum Likelihood phylogenetic tree, computed using the LG substitution model. A discrete Gamma distribution was used to model evolutionary rate differences with invariant sites. The analysis involved 20 amino acid sequences and a total of 297 positions (a), respectively 25 amino acid sequences and a total of 48 positions (b) in the final dataset. Bootstrap values > 70 are shown. Rhodobacter sphaeroides PufL sequence was used as an outgroup.