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. 2019 Dec 10;9:18676. doi: 10.1038/s41598-019-54814-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Discriminant analysis on the gene expression of 17 ion transport genes in S. pistillata. Each dot represents the gene expression profiles for each sample, and ellipses show the clusters. Blue denotes samples at 17 °C, yellow at 25 °C, and red at 32 °C. Closed dots (●) represent night individuals and open dots (○) represent day individuals. A, B and C show plots of Discriminant Factors (DF). DF1 explains 24% of variation, DF2 explains 24% and DF3 explains 22%. One-way ANOVAs carried out on each of the three axis of the discriminant analysis showed statistical differences between clusters (P < 0.05).