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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: J Immunol. 2019 Nov 15;203(12):3216–3224. doi: 10.4049/jimmunol.1900059

Figure 7. CD31 negatively regulates FcγR-mediated phagocytosis.

Figure 7.

PBM were nucleofected with either scrambled control siRNA (Scr) or siRNA against CD31, then cultured for 72 hours in the presence of M-CSF to promote cell survival. CD31 protein levels were measured using western blots. (A) Representative western blot of CD31 knockdown. Shown below is the densitometry measure of CD31 protein using calreticulin as loading control (n=7). (B) FcγR-mediated phagocytosis of fluoresceinated, IgG-coated SRBCs was evaluated after knockdown (n=7). Shown below are representative images showing phagocytosis in scrambled control versus CD31-knockdown samples. * p ≤ 0.05 between Scrambled-siRNA and CD31-siRNA samples.