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. 2019 Dec 10;9:18742. doi: 10.1038/s41598-019-55096-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of pS6 in OV1002 and HOX424 xenografts after treatment with PF-04691502 and gedatolisib. (A) pS6 (Ser235/236) expression detected by immunohistochemistry. Immunoscores are shown. (B) pS6 (Ser235/236) expression detected by immunofluorescence. Normalised AQUA scores are shown. (C) Examples of pS6 (Ser235/236) immunostaining in TMA cores. (D) Examples of pS6 (Ser235/236) immunofluorescence staining in TMA cores. Mean values shown are average values +/− S.D. for each group of xenografts. *P < 0.05 shown for ANOVA followed by Tukey post-test.