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. 2019 Dec 10;9:18729. doi: 10.1038/s41598-019-55239-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Expression of CD107a, a marker of degranulation, in mock (non-transduced) and CD19- CAR AB NK cells against against K562 (n = 12) a CD19 – cell line; Nalm-6 (n = 12), ALL cells (n = 11) and CLL cells (n = 9), all of them CD19 positive cells. (b) Expression of CD107a in mock (non-transduced) and CD19- CAR CB NK cells against K562 (n = 12) a CD19 – cell line; Nalm-6 (n = 10), ALL cells (n = 10) and CLL cells (n = 12). (c) A comparison of the cytolytic activity between AB CD19-CAR NK cells and CB CD19-CAR NK cells against K562 and CD19 expressing target cells. The bars represent the mean and error bars represent SEM. Two-Way ANOVA with multiple comparisons was used to analyze the data. p-Value: *p < 0.05, **p < 0.005, ***p < 0.001. ****p < 0.0001.